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Trinh Hoang Khai

Lab Technician

Trinh Hoang Khai

Trinh Hoang Khai

Phone: +32 9 264 60 69

Email: Hoangkhai.trinh@ugent.be

I am originally from a small village in the South of Vietnam where all habitants are involved in farming. I started my work at Cuu Long Delta Rice Research Institute in May 2000 as an assistant researcher on applying MAS (Maker Assisted Selection) techniques for rice breeding. I studied on blast resistance on rice using RFLP (Random Fragment Amplified Polymorphism) marker, using MAS following stress tolerance to saline conditions and high acid sulfate content in soil. I also worked on low phytic acid gene (lpa) through HIP (high inorganic phosphorous) for phenotype diversity and using SSR (Simple Sequence Repeats) maker. I finished my master’s thesis with title “Evaluation on somaclonal and gametoclonal variation of low phytic acid in rice Oryza sativa L”. Additionally, I had joined a program transfering techniques how to multiplicate rice varities, apply fertilizer, pesticides, fungicides on their own rice field. This program brought novel varities with high yeild and resistant pathogens or insects to farmers helping them replace new varieties planting in their farms to increase production. Hence I have fairly good experience with contacting Vietnamese farmers for dissemination activities. I gained this knowledge because of learning a subject named “Extension Method” at the university and working as an extension staff to investigate farmers activities at farming by staying, joining local traditional activies and  working with them in the field.

PhD research

I was awarded a scholarship by Vietnamese Government to undertaking PhD research in Gent University for four years since October 2013. The title of the study is Environmental Control Of Adventitious Rooting and Identification Of Underlying Hormone Signaling. In this study, we are researching on hormone signalling adventitous rooting to go insight into gene expression and finding candidate genes regulating adventitious roots in Arabidopsis hypocotyls.

Adventitious rooting (AR) contributes to asexual propagation as well as controlling abiotic response effectively. AR induction and initiation is studied through the central role of phyto-hormone indicating auxin. Here we are investigating a novel small molecule, hysparin, (hypocotyl specific adventitious root inducer) that has been shown to induce massive ARs in Arabidopsis thaliana.

Academic activities

  • 2019 – now: Postdoc, Scientific Collaborator for Projects: Bio2Bio, BioSunmulant, Bio4Food
  • 2013 – 2019: Ph.D. in Applied Biological Sciences – Ghent University
  • 2006 – 2013: Lecturer at CTEC, Can Tho Technical Economic College, Can Tho, Vietnam
  • 2003 – 2005: Msc in Biotechnology, Can Tho Uni., Can Tho Vietnam
  • 2000-2006: Assistant researcher at CLRRI, Cuu Long Delta Rice Research Institute, Can Tho, Vietnam
  • 1996 – 2000: Bachelor in Agronomy, Can Tho Uni., Can Tho Vietnam

Publications

  1. Zeng Y, Verstraeten I, Trinh HK, Heugebaert T, Stevens CV, Garcia-Maquilon I, Rodriguez PL, Vanneste S, Geelen D. Arabidopsis Hypocotyl Adventitious Root Formation Is Suppressed by ABA Signaling. Genes. 2021; 12(8):1141. https://doi.org/10.3390/genes12081141
  2. Trinh H.K., Verstraeten I., Geelen D. (2018) In Vitro Assay for Induction of Adventitious Rooting on Intact Arabidopsis Hypocotyls. In: Ristova D., Barbez E. (eds) Root Development. Methods in Molecular Biology, vol 1761. Humana Press, New York, NY. doi: https://doi.org/10.1007/978-1-4939-7747-5_7
  3. Babiychuk, E., Hoang, K. T., Vandepoele, K., Van De Slijke, E., Geelen, D., De Jaeger, G., … Kushnir, S. (2017). The mutation nrpb1-A325V in the largest subunit of RNA polymerase II suppresses compromised growth of Arabidopsis plants deficient in a function of the general transcription factor IIF. The Plant Journal, 89(4), 730–745. https://doi.org/10.1111/tpj.13417
  4. Trinh Hoang Khai and Nguyen Thi Lang. 2005. Using SSR marker to indentify allel vriation of somaclonals mutants in indica rice (Short communication). Omon rice 13. http://www.clrri.org/ver2/uploads/noidung/13-17.pdf
  5. Nguyen Thi Lang, T Hoang Khai, D Giang Tien, BT Duong Khuyeu, NV Tao, Nguyen Thi Lang, Nguyen Thi Xim, Trinh Hoang Khai and Bui Chi Buu. 2007. Exploit gametoclonal mutants of  F1 crosses in rice. Agriculture and Rural Development 7 (in Vietnamese).
  6. Nguyen Thi Lang and Trinh Hoang Khai. 2007. Develop rice somaclonal mutants of IR64 and OM1490 varieties. Agriculture and Rural Development 7 (in Vietnamese).
  7. Trinh Hoang Khai and Nguyen Thi Lang. 2006. Developing gametoclonals in rice for exploiting low phytic acid content. Agriculture and Rural Development 7 (in Vietnamese).
  8. Nguyen Thi Lang, Trinh Hoang Khai, Bui Thi Duong Khuyeu, Nguyen Van Tao, Hiromi Kobayashi and Bui Chi Buu. Genetic Variability of salt tolerance in rice. Proceeding of the 2002 annual workshop of JIRCAS Mekong Delta project. CLRRI (Nov. 26-28, 2002).
  9. Nguyen Thi Lang, Trinh Hoang Khai, Bui Thi Duong Khuyeu, Nguyen Van Tao, Hiromi Kobayashi and Bui Chi Buu. 2001. Genetic variability of salt tolerance in rice. Proceeding of the 2001 annual workshop of JIRCAS Mekong Delta project. CLRRI (Nov. 27-29, 2001).