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Haploidization

Haploidization and the role of translation in heat tolerance

PhD project Cedric Schindfessel

Natural variation in temperature sensitivity of male meiosis and pollen development

Sexual reproduction creates genetic variation in offspring that through natural selection results in species adapted to changing environments. Meiosis, the process that underlies genetic variation, is itself influenced by several environmental factors and appears to be highly sensitive to temperature stress. High and low temperatures or sudden temperature changes during meiosis can disrupt the normal process, often resulting in decreased fertility. In plants, heat stress causes changes in gene expression and chromosome structure, defects in cytoskeletal organization and cytokinesis. Given the importance of good fertility for grain production and the survival of natural populations, it is vital for future food production and biodiversity that we understand how climate change affects meiosis in plants. This project studies the natural variation in temperature stress sensitivity, in order to identify the genetic factors and alleles responsible for defects in male meiosis and reduced fertility in both Arabidopsis and rice due to temperature stress.

Tetrad stage meiocytes from Arabidopsis thaliana Ler and Mt-0 incubated for 24h or 32°C. Microspores stained with orcein (panels on the left) and aniline blue staining (panels on the right).

PhD project Limin Sun

Genetic factors required for pollen development

The PhD project of Limin is to investigate novel genes involved in the viability and fertility of pollen in Arabidopsis thaliana. Genes unique to plants are required for pollen development and when mutated, a gametophytic lethality occurs. Such genes are of interest to unravel molecular processes that underly cell division and cell differentiation.